In patients who undergo renal transplantation urinary tract tumors have an incidence of approximately 1.5%-3.3%. We report a rare case of renal cell carcinoma occurring in a transplanted kidney 25 years after the transplant. The lesion was treated by CT-guided percutaneous cryoablation with the ablation of renal lesion. This approach offers more accuracy compared with ultrasonography (US), and it is faster compared to magnetic resonance (MR) guidance. In transplanted kidneys CT-guided cryoablation seems to be safe.