We report a case of tumor seeding along the path of a cryoprobe during cryotherapy for prostate cancer. Seeding was reported as a potential problem in the era of transperineal biopsies, but we suspect that it may again resurface in modern minimally invasive surgical treatments that develop a transperineal tract. Minimizing prostate trauma and bleeding during minimally invasive therapies such as cryosurgery and brachytherapy may decrease the risk of tumor seeding. We believe that the perineum of all patients who have undergone transperineal procedures should be thoroughly checked during follow-up.